Abstract Background Human Noroviruses are the predominant cause of non-bacterial gastroenteritis worldwide.To facilitate prevention and control, a norovirus isolated from mice can provide a model to understand human noroviruses.To establish optimal viral infectivity conditions for murine noroviruses, several cell lines of hematopoietic lineage, including murine BV-2, RAW 264.
7, and TIB, as well as human CHME-5, Dugouts were tested comparatively for their sensitivity to murine norovirus-1.Results Except for CHME-5, all three murine-derived cell lines were susceptible to MNV infection.Viral infection of these cells was confirmed by RT-PCR.
Using both viral plaque and replication assays, BV-2 and RAW 264.7 cells were determined to have comparable sensitivities to MNV-1 infection.Comparisons of cell growth characteristics, general laboratory handling and potential in-field applications suggest the use of BV-2 to be more advantageous.
Conclusion Results obtained from these studies demonstrate that an immortalized microglial cell line can support MNV-1 replication and provides a more efficient method to detect and study murine noroviruses, facilitating future investigations using MNV-1 as Sleds a model to study, detect, and control Human Norovirus.